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Effect of nitric oxide synthase inhibitor L- NAME on the induction of brain ischemic tolerance in rats

LIU Hui- Qing, LI Wen Bin, FENG Rong Fang, LI Qing Jun, CHEN Xiao-Ling, ZHOU Ai- Min, ZHAO Hong Gang, Ai Jie

Department of Pathophysiology，Institute of Basic Medicine，Hebei Medical University，Shi jiazhuang050017

Abstract

To explore the role of NO in the induction of brain ische mic tolerance(BIT) in vivo，the effect of nitric oxide synthase(NOS) inhibitor L- NAME on the induction of BIT induced by cerebral ische mic preconditioning(CI P) was investigated in the hippocampal CAI subfield in CI P and ischemic insult models established rat four vessel occlusion using brain tissue section and thionine staining methods·Fifty-four male Wistar rats were divided into 6 groups:(1)sham-operated group(n一6):bilateral common arteries were separated without oceluding the cerebral blood flow;(2) ische mia group(n一6):an ische mic insult for 10 min was given;(3) CI P+ische mia group(n一6):3- min CIP was preformed 72 h prior to 10- min ische mic insult;(4) L- NAME groul (total n= 24，n = 6 for each suroup):L- NAME(5 mg/ kg，i. p.)was administered 1 h prior to CIP and 1 12 and 36 h after CI P，respectively .Other procedures were the same as those for the CIP+ische mia group;(5)L- NAME+L- Arg group(n=6):L- NAME(5 mg/ kg，i. p.)and L- Arg(300 mg/ kg，i. p.)were administered 1 h prior to CIP，other procedures were the same as those for the L- NAME group;(6) L- NAME+is the mia group(n一6):L- NAME(5 mg/ kg，i. p.)was administered 72 h before the 10- min ischemic insult.

Key words: neurobiology;ische mic preconditioning;Nitric oxide;L- NAME;Hippoca mpus;rats

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Citing This Article：

LIU Hui- Qing, LI Wen Bin, FENG Rong Fang, LI Qing Jun, CHEN Xiao-Ling, ZHOU Ai- Min, ZHAO Hong Gang, Ai Jie. Effect of nitric oxide synthase inhibitor L- NAME on the induction of brain ischemic tolerance in rats. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).