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A preliminary observation on the hemopoietic inductive effect of marrow stroma

Ji Sunquan, Du Zhenging, Jin Shuzi, Wang Shangin, Cao Xouzhen

The Genetal Hospital of the Ministry of Astronautry

Abstract

The adherent cell layer formed by cultivating bone marrow cell in a liquid
system in dishes was taken as a stromal layer (SL) for studying the interaction of the hemopoietic cells and the stroma. The granulocyte-macrophage progenitor
cells (CFU-C) culture system in agar was overplated directly onto the human and mouse marrow SL which has been incubated for 1 to 3 weeks. In comparison with the CFU-C grown without SL as a control, the CFU-C grown on the first week SL were 51.07±3.540o (human) and 27.30±4.6800 (mouse) respectively; on the second week SL, the growth of CFU-C was practically the same; while the sum of the CFU-C colonies overlayed onto the third week SL increased up to 150.70±17.63%(human), and 146.89±10.01% (mouse).The results showed that SL affected granulopoiesis from inhibition to stimulation in a period of 3 weeks. This affection was probably mediated by the colony stimulating factor.

Key words: Stroma layer;CFU-C;Haemopoiesis;Amisodamin

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Citing This Article：

Ji Sunquan, Du Zhenging, Jin Shuzi, Wang Shangin, Cao Xouzhen. A preliminary observation on the hemopoietic inductive effect of marrow stroma. Acta Physiol Sin 1987; 39 (2): (in Chinese with English abstract).