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[Regulation of pyloric rhythm by I(A) and I(h) in crayfish stomatogastric ganglion.] [Article in Chinese]

KUANG Guo-Hui, LIU Yi-Hui^*, REN Wei

College of Life Sciences, Shaanxi Normal University, Xi’an 710062, China

Abstract

The stomatogastric ganglion (STG) of shellfish includes 30 neurons and produces pyloric rhythms. It is the common model to study central pattern generator (CPG). Regulation of pyloric rhythms not only is related to the property of single neurons in STG but also depends on the connections and property of the whole neuronal network. It has been found that transient potassium current (IA) and hyperpolarization-activated cation current (Ih) exist in certain types of neurons of STG. However, roles played by these two currents in maintaining and regulating the pyloric rhythms are unknown. In the present study, in vitro electrophysiological recordings were performed on crayfish STG to examine the role played by IA and Ih in regulation of pyloric rhythm. 4AP (2 mmol/L), a specific inhibitor of IA, caused a decrease in pyloric cycle (P < 0.01), an increase in PD (pyloric dilator) ratio, a decrease in PY (pyloric) ratio (P < 0.01) and delay of phases of LP and PY firing. ZD7288 (100 μmol/L), a specific inhibitor of Ih, caused a decrease in pyloric cycle (P < 0.01), an increase in PD ratio (P < 0.01), an increase in LP (lateral pyloric) ratio (P < 0.01), a decrease in PY ratio (P < 0.01) and delay of phases of LP and PY firing. These results indicate that IA and Ih play important roles in regulating pyloric rhythms in crayfish STG.

Key words: central pattern generator; stomatogastric ganglion; pyloric rhythm; 4AP; ZD7288

Received: 2011-12-28　　Accepted: 2012-02-20

Corresponding author: 刘一辉　　E-mail: liu.y.hui@gmail.com

Citing This Article：

KUANG Guo-Hui, LIU Yi-Hui, REN Wei. [Regulation of pyloric rhythm by I(A) and I(h) in crayfish stomatogastric ganglion.] [Article in Chinese]. Acta Physiol Sin 2012; 64 (3): 275-281 (in Chinese with English abstract).