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The changes of bcl-2、bax expression and neuron apoptosis in the hippocampus after the blockade of cervical lymphatics of rats

Li Yuxian, Xian Zuoli, Chen Lianbi, Ye Wenjing, Yang Mingfeng, Sun Qiaoling

Institute of Physiology, Shandong University School of Medicine.Jinan 250012,Shandong；China

Abstract

To investigate the changes in bcl-2、bax expression and neuron apoptosis in the hippocampus after the blockade of cervical lymphatics, the model of lymphostatic encephalopathy was established by occluding and removing both the superficial and deep cervical lymph nodes in rats. The animals were sacrificed at 1, 2, 3, 5, 7 and 14 days after operation. H＆E staining was used to observe the structure of brain tissues and TUNEL staining was used to detect in situ cell apoptosis in the hippocampus. The expression of bcl-2 and bax in the hippocampus were examined by RT-PCR. The results showed that cerebroedema appeared at day 2 and was most serious at day 5 after the blockade of cervical lymphatics. The number of TUNEL positive cells began to increase at day 2 and reached a maximum at day 5. The expression of bax began to increase at day 1 and reached a maximum at day 2. The expression of bcl-2 began to decrease at day 1 and dropped to minimum at day 5. The items mentioned above recovered to control level at day 14. These results suggested that lymphostatic encephalopathy following the blockade of cervical lymphatics resulted in changes in bcl-2 and bax expression in the hippocampus and that apoptosis was the main form of neuron death.

Key words: Rats, Wistar;Lymph node excision;Brain injuries;Apoptosis;Genes, bcl-2 ;

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Citing This Article：

Li Yuxian, Xian Zuoli, Chen Lianbi, Ye Wenjing, Yang Mingfeng, Sun Qiaoling. The changes of bcl-2、bax expression and neuron apoptosis in the hippocampus after the blockade of cervical lymphatics of rats. Acta Physiol Sin 2005; 57 (1): (in Chinese with English abstract).