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Vasorelaxing role of vasonatrin peptide in human intramammary artery in vitro

Yu Jun, ZHU Mao Zhang, WEI GengZen, CHEN Bao Yin, LU Shun Yan, Kang Yunfan, Guo Hai Tao, MA Heng, Dong Mingqing

Department of Physiology Radiology Dpartment of Tangdu Hospital,Cardiovascular Surgery Center of Xijing Hospital and Department of Pathophysiology, Fourth Military Medical University Xi'an 710032

Abstract

The purpose of this study was to investigate the vasorelaxing effect of vasonatrin peptide (VNP) on human intramammary artery(HI MA) .The vasorelaxing effect of VNP on HI MA was measured by means of perfusion in vitro .The effects of HS-142-1，TEA，8- Br c G MP and methylene blue (MB) were also observe d·It was found that VNP caused a concentration- dependent relaxation in HI MAwhich was independent of the endothelium .8-BrcGMP(0. 1一1 000 p mol/ L) also caused a concentration dependent relaxation in HI MA.The vasorelaxing effect of VNP disappeared in the presence of HS142-1(20 p mol/ L)，an antagonist of the natriuretic peptide guanylate cyclase(GC) receptor·MB(10
p mol/ L)，an inhibitor of GC，not only blocked completely the relaxation of HI MA，but also enhanced the vascular contraction induced by orepinephrine .TEA(1 m mol/ L)，an antagonist of calcium activated potassium channels(Kca)，reduced but not completely blocked the vasorelaxing effect of VNP .These
findings suggest that VNP can relax HI MA，which is independent of the endothelium .This effect is possibly achieved by the binding of VNP with the natriuretic peptide GC receptors in the smooth muscle cells (S MCs)，leading to an increase in intracellular cGMP level.Moreover，the vasorelaxing effect of VNP is associated with Kca.

Key words: vasonatrin peptide;human intra ma m wary artery;cGMP;;TEA;

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Citing This Article：

Yu Jun, ZHU Mao Zhang, WEI GengZen, CHEN Bao Yin, LU Shun Yan, Kang Yunfan, Guo Hai Tao, MA Heng, Dong Mingqing. Vasorelaxing role of vasonatrin peptide in human intramammary artery in vitro. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).