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Membrane estrogen receptor mediates the rapid nongenomic activation of endothelial nitric oxide synthase by estrogen

WANG Ting Huai, FU Xiao- Dong, Yang Dan, Tan Zhi, PAN Jing Yun

Department of Physiology，Sun Yat-sen University of Medical Sciences，Guangzhou 510080

Abstract

In the present study，confluent bovine aortic endothelial cells(BAECs) were used to study the rapid nongenomic effects of 17f}-estradiol and the membrane impermeable conjugated 17f}-estradiol (EZBSA) on the activation of endothelial nitric oxide synthase(eNOS) and mitogen activated protein kinase(MAP K) .eNOS activation was assessed in whole cells by measuring[3H]L-arginine conversion to [3H]L- citrulline .MAPK activity was determined by Western Slotting .The results obtained show that the addition of various concentrations of EZ(0. 001一1 p mol/ L) resulted in 122士29，186士17，83士20
and 157士29%increases in eNOS activity，respectively，in BAECs within 15 min of exposure to the hor mone .E2(0-01 p mol/ L)一stimulated eNOS activity was detectable during 5-，15- and 30- min incubation which yielded increases of 37士6，56士9 and 38士8%，respectively .The increase reached a plateau from
15 through 30 min and rapidly declined thereafter.EZ BS A(17. 5 ng/ ml) also enhanced eNOS activity by an increase of 35士9%above the basal activity .The effect of EZ and EZBSA on eNOS activation was unaffected by actinomycin D(25 pg/ ml) but was obviously inhibited by tamoxifen(0.1 p mol/ L) and
PD98059(50 p mol/ L).Compared with control，EZ and EZBSA sti mulation of BAECs for 1 5 min caused an increase in MAP K activity by 428士17 and 360士14%respectively.This effect was blocked by tamoxifen.These results suggest that there might be the me mbrane estrogen receptor localized on BAECs，
which mediates the rapid nongenomic effect of estrogen on eNOS activation through MAPK pathways.

Key words: Estrogen;me mbrane estrogen receptor;nitric oxide synthase;mitogen activated protein kinase;vascular endothelial cell

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Citing This Article：

WANG Ting Huai, FU Xiao- Dong, Yang Dan, Tan Zhi, PAN Jing Yun. Membrane estrogen receptor mediates the rapid nongenomic activation of endothelial nitric oxide synthase by estrogen. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).