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Charges in the phosphorylation of cAMP response element binding protein in the rat nuclets accumbers after ache anal chrornc ethaml admirisstration

Li Jing, LI Yue- Hual, ZHANG Xiao Hu, ZHU Xue Jiang, GE Ying Bin, Yuan Xiaoru

(Department of Pathology and Pathophysiology and Department of Physiology, Nanjing Medical University，Nanjing 210029l

Abstract

To define the molecular basis of ethanol dependence，changes in the phosphorylation of cAMP response element binding protein(CREB) in the nucleus accu m阮ns of rats after acute and chronic ethanol administration were detected using immunohistochemist ry.The results demonstrate that the expression of phospho-CREB(p-CREB) protein in the rat nucleus accumbens significantly increased after 15 min of acute ethanol exposure，reaching a peak at 30 min after ethanol administration .The increment remained after 1 or 6 h of ethanol exposure compared to the control rats.In contrast，chronic intake of ethanol solution obviously decreased the expression of p-CREB protein compared to the control rats .The decrement remained 24 h or 72 h after ethanol withdrawal，and returned to the control levels after 7 d of
ethanol withdrawal .The results suggest that an acute ethanol administration led to an increase in the phosphorylation of CREB in the nucleus accumbens，but chronic ethanol administration produced a decreme nt，which is possibly one of the molecular mechanisms of alcohol dependence.

Key words: Ethanol;nucleus accu mbens;cAMP response element binding protein(CREB)

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Citing This Article：

Li Jing, LI Yue- Hual, ZHANG Xiao Hu, ZHU Xue Jiang, GE Ying Bin, Yuan Xiaoru. Charges in the phosphorylation of cAMP response element binding protein in the rat nuclets accumbers after ache anal chrornc ethaml admirisstration. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).