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Regulatory peptides modulate I CAM 1 gene expression and NF #kappa#B activity in bronchial epithelial cells

TAN Yu Rong, QIN Mao- Qun, GUAN Cha Xiang, ZHANG Chang Qing, LUO Zi Qiang, SUN Xiu Hong

Department of Physiology，Xiangya School of Medicine，Central South University，Changsha 410078

Abstract

Intercellular adhesion molecule-1(ICAM-1)is an important adhesion molecule leading to adhesion between cells;NFKB，being universally distributed in the organism，is an important nuclear transcription factor leading to a rapid response to the stimuli .Line of evidence have shown that ICAM-1 transcription and NF-KB activation is an important step of inflammatory reaction.To test that intrapulmonary regulatory peptides modulate inflammatory lesion of bronchial epithelial cells(BECs) through their effect on ICAM-1 expression and nuclear factor KB(NF- KB) activation，we used immunocytochemistry，RT PCR, and electrophoretic mobility- shift assay(EMSA) to determine the ICAM-1 expression and NF-KB activity in BECs.The effects of NF-KB inhibitor MG 132 on ICA1vF1 expression were also observed.The results showed that vasoactive intestinal peptide(VIP) and epidermal growth
factor(EGF) decreased ICAM-1 expression in O3-stressed BECs，while endothelirrl(ET-1)and calcitonin gene related peptides (CGRP) increased ICAIYFI expression in resting BECs.MG132 blocked ICA1vF1 expression induced byq，ET-1 and CGRP.
The results obtained by using EMSA confirmed that VIP and EGF restrained the activation of NF-KB inq一stressed BECs;CGRP and ET -1 promoted activation of NF-KB.These observations indicate that VIP and EGF abated the injury by means of dowrr regulatory effects on ICAM-1 transcription and NF-#Kappa#B activation，while ET -1 and CGRP enhanced the inflammation reaction by an up regulatory effect .It is suggested that a developing and intensive airway inflammation correlates closely with a persistent expression of ICAIYFI and repeated activation of NF- #Kappa#B.

Key words: intrapulmonary regulatory peptides;intercellular adhesion molecule-1(ICAM-1);nuclear factor KB(NF-KB);hrnnchial Pithpfal PPllc

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Citing This Article：

TAN Yu Rong, QIN Mao- Qun, GUAN Cha Xiang, ZHANG Chang Qing, LUO Zi Qiang, SUN Xiu Hong. Regulatory peptides modulate I CAM 1 gene expression and NF #kappa#B activity in bronchial epithelial cells. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).