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Charges in the gene expression profile of the left heart verntricle duriing growth in the rat

Li Ping, LI Jin Liang, H OU Rong, HAN QI-DE, ZHANG You Yi

Institute o f Vascular Medicine，Pe ki二又University Third Hospital，The Key Laboratory of EducatiMinistry on Molecular Cardiology，Beijing 100083o

Abstract

Wistar rats of 8，10 and 12- week old were chosen for study of the relationship between car diac growth and its gene expression profile changes during maturation .The ultrasonic parameters of rat hearts were recorded before sacrifice，then total RNA of left ventricle were extracted and gene expression profiles were analyzed by cDNA microarray .During growth from 8 weeks to 12 weeks，the body weight increased by 45.5(287士13 g vs 197士10 g)，and the increment in the first two- week period was equal to that of the second two- week period.The mass of left ventricle and the posterior wall thickness increased by 27. 7%(0.60士0. 03 g vs 0.47士0. 02 g) and 23. 6%(2. 04士0.04 mm vs 1 .65士0.13m m)，respectively，and their increment in the first two- week period was much more than that in the secand one .Meanwhile，the gene expression profile of the left ventricle changed significantly，which in-
volved cellular structure，metabolis m，oxidative stress，signal transduction，etc .Compared with the 8week-old rats，these genes were mostly up- regulated in 10- week old rats，while for 12- week old rats，the gene expression profile of the left ventricle recovered to the pattern of 8- week old rats again on the
whole .These results suggest that the relationship between the changes in cardiac function and gene expression profile can be analyzed comprehensively with the technique of microarray，and that the changes in gene expression profile of the left ventricle during rat maturation adapt to the physiological growth of heart，which is of benefit for keeping the metabolis m balance between materials and energy·

Key words: physiology;Gene expression;microarray;Heart;growth

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Citing This Article：

Li Ping, LI Jin Liang, H OU Rong, HAN QI-DE, ZHANG You Yi. Charges in the gene expression profile of the left heart verntricle duriing growth in the rat. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).