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Influence of ACh on the level of protein kinase C，intracellular free Ca~(2+)and cyclic AMP/ cyclic GMP of cultured human pituitary adenoma cells

CHI Su Min, LI Cheng Xin, LIU Ya-Li, ZHU Yun Long, GU Jian Wen

Department of Physiology, Center of Dermatology of Chinese PLA, Xijing Hospital and Department of Neurosurgery, Xijing Hospital, the Fourth Military Medical University,Xi'an 710032

Abstract

We found previously that ACh can significantly inhibit the proliferation of cultured human pituitary adenoma cells .In order to make a further investigation of the mechanism of the inhibitory effect of ACh on the proliferation of pituitary adenoma cells，we observed the levels of protein kinase C(P KC)，[Cat~(+)]，and cAMP/cGMP in cultured pituitary adenoma cells after treatment with ACh.The results demonstrate that(1)compared with control，P MA，a P KC activator，increased the activity of cytoplas m，membrane and total PKC in human pituitary adenoma cells .However，after a 15- min treatment
with ACh(10 p mol/ L)，a significant reduction of the activity of cytoplasm，me mbrane and total P KC in human pituitary adenoma cells was observed，and the reduction effect could be blocked by atropine.(2)The level of[Ca~(2+)]，of single adenoma cells was found to decrease immediately on the addition of ACh
(10 pmol/L)，which could also be blocked by atropine.(3) ACh increased the amount of cAMP in the cytoplasm of human pituitary adenoma cells，but had no effect on that of cGMP.These data provide an important clue to explore the molecular mechanisms of the inhibitory effect of ACh on the proliferation of
pituitary adenoma cells，and suggest that the modulating effect of ACh on the proliferation of pituitary adenoma cells results from the interactions of several cellular signaling pathways.

Key words: ACh;pituitary adenoma;P KC;[Ca~(2+)]i;

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Citing This Article：

CHI Su Min, LI Cheng Xin, LIU Ya-Li, ZHU Yun Long, GU Jian Wen . Influence of ACh on the level of protein kinase C，intracellular free Ca~(2+)and cyclic AMP/ cyclic GMP of cultured human pituitary adenoma cells. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).