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Influence of nitric oxide on the angiotensin Ⅱ一activated protein kinase C activaty in cultured neonatal rat cardiomycytes

FU SHi-GAN, XIE Xie- Ju, JILi- Min, LIU Pei Qing, PAN Jing Yun, Lu Wei

Department of Physiology, Hainan Medical College, Haikou 571101；Department of Physiology Sun Yat-Sen University of Medical Sciences，Guangzhou 510089

Abstract

We examined the effect of endogenous and exogenous nitric oxide(NO) on protein kinase C(PKC) activity induced by angiotensin Ⅱ(Ang Ⅱ)in cultured neonatal rat cardiomyocytes .The results are as follows .The activity of PKC was increased by Ang Ⅱ(0. 01一10 p mol/ L) in a dose-dependent manner，but decreased by NO precursor L一arginine(L一Arg)(10 umol/ L一1 0 m mol/ L) in a
dose-dependent manner in cultured neonatal rat cardiomyocytes .Pretreatment with L一Arg(100 umol/L) decreased significantly Ang 1I一activated PKC activity and PKC activity induced by phorbol 12myristate 13-acetate(P MA)(10 pmol/L)，a PKC activator.Pretreatment with N(}-nitro- L-argingie methyl ester(L一NAME)，a nitric oxide synthase(NOS) Mocker，may inhibit significantly the role of
L一Arg on Ang Ⅱ一and P MA activated P KC activity .The activity of PKC was also decreased by NO donor sodium nitroprusside(S NP)(10 umol/L一1 mmol/ L) in a dose-dependent manner in cultured neonatal rat cardiomyocytes .Pretreatment with SNP(1 0 p mol/ L) decreased significantly AngⅡ一and P MA activated P KC activity.These results indicate that PKC was controlled by both NO and Ang Ⅱ.
PKC may be a“cross talk" between Ang Ⅱ and NO in cardiomyocytes .NO abolished the activity of PKC and impaired PKC downstream signaling transduction pathway cascades.

Key words: neonatal rat myocardial cell culture;angiotensinⅡ;;

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Citing This Article：

FU SHi-GAN, XIE Xie- Ju, JILi- Min, LIU Pei Qing, PAN Jing Yun, Lu Wei. Influence of nitric oxide on the angiotensin Ⅱ一activated protein kinase C activaty in cultured neonatal rat cardiomycytes. Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).