Issue Archive

Effect of hyproxia on membrane potential and input resistance of cultured carotid body glomus cells of rat

He Shefang, Wang Lihua

Shanghai Brain Besearch Institute, Chinese Acadmey of Science. Shanghai 200031, China；Shanghai Institute of Physioloyg, Chinese Academy of Sciences. Shanghai 200031, China

Abstract

Carotid bodies were removed from rats (50-100g) under sodium pentobarbitone
anesthesia and dissociated by mechanical means to obtain small clusters and single glomus cells to be cultured under normoxic[5%CO_(2),20%O_(2),75%N_(2), 37℃) or by poxic[5%CO_(2),10%O_(2),85%N_(2),37℃)conditions for 3-5 days. They are respec tively called normoxic or hypoxic cells.The membrane potential (MP)and input resistance (Ri)of the normoxic cells were measured under normoxic condition as controls. The results show that MP of the normoxic cells measured under hypoxic condition increased in about half the cells and dicreased in an other half.The percentage of cells with increased MP was higher under 100%N_(2) than under hypoxia. The MP and Ri of the hypoxic cells measured under nor moxia were not significantly different from the control values，but the MP and Ri of the hypoxic cells measured under hypoxia were significantly higher than those of the control (MP:P<0.01，Ri:P<0.05). The results suggest that oxygen sensing of the carntid body is raflected by changes in MP and Pi of the glomus cells.

Key words: Carotid body;Glomus cell;Culture;membrane potential;Hypoxia

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

He Shefang, Wang Lihua. Effect of hyproxia on membrane potential and input resistance of cultured carotid body glomus cells of rat. Acta Physiol Sin 1993; 45 (1): (in Chinese with English abstract).