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The role of activation of nuclear factor kappa B of rat brain in the pathogenesis of experimerntal allergic encephalomyelitis

TAN Gua Jun, YANG Tiair Zhu, ZHAO Mao yun, ZHOU Li-Ma, CAO Cur Li, MA Chang Sheng

Department of Neurobiology and Pathophysiology, Hebei Medical University, Shijiazhuang 050017

Abstract

To investigate the role of activated nuclear factorxB(NF-KB) in experimental allergic encephalo myelitis(E AE)，the activity and protein expression of NF-KB p65 in rat brain tissues，which were extracted from EAE rats at 1，7，1 4 and 21 d respectively after EAE was induced by CFA- GPSCH，
were measured with electrophoretic mobility shift assay and unohistochemistry.The relationship Setween activated NF-KB and symptoms of EAE was also investigated.The results showed that protein expression level and the activity of NF-KB were very low in the brain of the control group .After EAE was induced，the activity of NF-KB and the level of the protein expression in the brains increased gradually with the development of symptoms and brain pathology of EAE.On d 14，both the activity and the level of protein expression in the brains reached a peak，the positive cells of NF-KB were mainly located at the choroid plexuses and subfornical organ，as well as around the regions of“sleeve-like" lesion foci，which were coincident with the locations of lesions of EAE .The incidence，symptoms，reduction of the body
weight and pathology of EAE rats brains at the above locations were most significant .On d 21 the activity of NF-KB and level of the protein expression reduced gradually，which was in parallel with a gradual alleviation of the symptoms of EAE rats .After a specific inhibitor of NF-KB，PDTC was applied，the symptoms and pathological lesions of EAE rat brain were mitigated markedly .The above results indicate that the dynamic changes in the activity and protein expression of NF-KB were in parallel with the changes in symptoms and pathological lesion of EAE rat brains .In conclusion，the activated NF-KB in the brain may play a critical role in the pathogenesis of EAE，and application of some inhibitors of NF KB，such as PDTC，may be one of the effective therapeutic methods for prevention and treatment of EAE.

Key words: neurobiology;encephalo myelitis;electrophoretic mobility shift assay;NF kappa B;subfornical organ

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Citing This Article：

TAN Gua Jun, YANG Tiair Zhu, ZHAO Mao yun, ZHOU Li-Ma, CAO Cur Li, MA Chang Sheng. The role of activation of nuclear factor kappa B of rat brain in the pathogenesis of experimerntal allergic encephalomyelitis . Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).