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Tissue specific expression of、Na~(+)-H~(+) exchanger isoformas at two developmental stages of human fetus

LIN Wair Mn, CHEN Xiair Hua, XU Ro, LI U Xuan, XU Ping

'Laboratory of Genomic Physiology and Center for Brain Research, Liren Laboratory, School of Life Sciences Fundan University, Shanghai 200433；School of Life Sciences , China Easten Normal University, Shanghai 200062

Abstract

Na~(十)-H~(十)exchangers(NHE) are major membrane proteins that have been identified as signal transduction mediators in the regulation of cell differentiation and important me mbrane ion transporters in the regula-
tion of the intercellular pH and the cell volume. NHE are composed of at least six isoforms and activated in growth factor regulated cell rentiation.However，little is known about the differential regulation of NHE expression in the development·In the present study，we studied developmental regulation of the expression of NHE isoforms in human fetal tissues by comparing the expression of various isoforms between two developmental stages，i. e.，week 11(11w and week 16(16w .The results demonstrated that NHEl transcripts were ex-
pressed ubiquitously .In comparison to the expression at 16 W，the level of NHEl transcripts was low and varied significantly in a tissue-specific pattern at 11 W，suggesting that the“house-keeping" function of MHEl occurs at
11 W or earlier and becomes well established at least as early as at 16 W.The tissue-specifically restricted expression of NHE2 and NHE3 was regulated at 11 W and 16 W in an opposite tendency，supporting the overlapping relationship between NHE2 and NHE3 in the tissue distribution as reported in adults .NHE5 expression was relatively ubiquitous at 11 W and became restricted in the cerebellum at 16 W，suggesting that the restrictive expression of NHE5 in the brain occurs later than that of other isoforms .The present study demonstrates a space time-dependent regulation of the tissue-specific expression pattern of NHE isoforms during human development between 11 W and 16 W.The results also suggest that at 16 Wor earlier the expression pattern of developing tis-
sues becomes similar to that of adult tissues.The observed developmental regulation of NHE expression provides a molecular basis for further study of the function and regulation of NHE gene during development.

Key words: Physiology;Gene expression;RT PCR; Na~(十)-H~(十)exchangers

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Citing This Article：

LIN Wair Mn, CHEN Xiair Hua, XU Ro, LI U Xuan, XU Ping. Tissue specific expression of、Na~(+)-H~(+) exchanger isoformas at two developmental stages of human fetus. Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).