Issue Archive

Preparation and inderntification of polyclonal antiserum against arngiotensi noggin

MENG Qing- Jun, Li Jian, ZHANG Wen Hong, ZHANG Le-Ning, ZHANG Li Fan

Department of Aerospace Physiology and Department of Biochemistry and Molecular Biology, the fourth Military Medical University, Xi'an 710032

Abstract

For studying the expression and distribution of angiotensinogen(AGT)，the C to minus of rat AGT gene was expressed in E. coli .Rabbits were immunized with expressed AGT protein and sera from different rabbits were raised.ELISAshowed a high titre(125 600) of the antiserum.With the antise ru m，Western blotting recognized not only the prokaryotic expressed AGT，but also the endogenous AGT protein in liver tissue of both rats and humans .Using this antiserum，i m munohistoche mistry showed the expression of AGT protein in islet cells of human pancreas as well as in epithelium of human
bile duct .These results suggest that the prokaryotic expressed AGT protein is an effective immunogen for the preparation of anti-AGT antiserum .Our present work provides an important tool for study of the path ophysiological role of AGT as well as local renixr angiotensin system.

Key words: angiotensinogen;prokaryotic expression;antiserum;specificity

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

MENG Qing- Jun, Li Jian, ZHANG Wen Hong, ZHANG Le-Ning, ZHANG Li Fan. Preparation and inderntification of polyclonal antiserum against arngiotensi noggin. Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).