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Pituitary adenyate cyclase activating polypeptide protects neura 2 a cells from #beta# amyloid protein cytotoxicity by modulating intracellular calcium

GUI Law Runi, ZHOU Yang, ZHANG Bingr Lie, Li Wenbin

Institute o f Microbiology, Beijing Military Medical College, Beijing 100071

Abstract

MTT analysis and intracellular calcium measurement by using confocal laser scanning microscopy were used to study the possible mechanism of protective effect of pituitary adenylate cyclase activating polypeptide 27(PACAP27) from p amyloid proteinA_(#beta#)induced neurotoxicity.The results
showed that treatment with PACAP(less than 0. 1 p mol/ L) increased the survival and reproductive aSility of neuro-2a cells and protected the neuro-2a cells from being injured byA_(#beta#)The protective effect of PACAP27 was reversed by the competitive PACAP receptor antagonist PACAP6-27 .An increase in intracellular calcium was observed when the cells were challenged withA_(#beta#)and PACAP·But the calcium increase induced byA_(#beta#)kept stable for a long time while PACAP caused a transient rise in intracellular calciu m.The intracellular calcium increase induced byA_(#beta#)was blocked by pretreatment with PACAP for 10 min.It is suggested that the neuroprotective effect of PACAP against neuronal damage induced byA_(#beta#) may result from its role in inhibiting the sustained rise in intracellular calcium.

Key words: p amyloid protein;pituitary adenylate cyclase ;;;

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Citing This Article：

GUI Law Runi, ZHOU Yang, ZHANG Bingr Lie, Li Wenbin. Pituitary adenyate cyclase activating polypeptide protects neura 2 a cells from #beta# amyloid protein cytotoxicity by modulating intracellular calcium. Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).