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Calbindin D28K containing neurons receivirng visceral and somatic nociceptive information in interstitial nucleus of the spinal trigeminal tract project to the parabrachial nuclei in the ratt

MA Weir Ling, ZHANG Weir Bin, ZHANG YuFei

Department of Anatomy and K. K. Leung Brain Research Centre，the Fourth Military Medical University，Xi an 710032-

Abstract

The calbindin D 28 k(CB)一containing neurons in the interstitial nucleus of the spinal trige minal tract(INV) that receive visceral and orofacial somatic nociceptive information and emanate projections to the parabrachial nuclei(PB) were investigated by the triple-labeled methods of fluorogold
(FG) retrograde tracing combined with Fos and CB proteins immunofluorescence histochemistry in the rat .The results showed(1)in the perioral stimulation group，a large number of FGretrograde labeled and Fos-i m munoreactive neurons were found in the paratrige urinal nucleus(Pa V) and the dorsal para-
marginal nucleus(PaMd) of the INV ipsilateral to FG and formalin injection made to the PB and lips，respectively，while a lot of CB-immunoreactive neurons were distributed in the INV bilaterally;(2) amajority of the FGretrograde labeled neurons(77. 3%)were double-labeled with CB，and 40. 7%of
them were double-labeled with Fos;about 38.5%of FG/ CB double-labeled neurons were FG/ CB/ Fostriple-labeled in the INV;and(3) in the upper alimentary tract stimulation group，the distribution and the numbers of FG-retrograde labeled，C& i m munoreactive neurons and F G/ CB double-labeled neurons in
the INV were similar to those of the perioral stimulation group as described above，except that the Fos immunoreactive neurons were distributed in the INV bilaterally，approximately 41·9%of the FGretrograde labeled neurons were FG/ Fos double-labeled，and over half(52. 0%)of those double-labeled neurons were FG/ CB/ Fos triple-labeled.The results indicate that a part of CB-containing neurons in the INV receive orofacial somatic and visceral nociceptive information and that these neurons sent projections directly to the PB .The C什containing neurons might play an important role in the transmission of the
peripheral nociceptive information from INV to PB.

Key words: eurobiology;interstitial nucleus of the spinal trige urinal tract;im munohistoche mistry; parabrachial nuclei;nociceptive information;calbindin仆28k;rat

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Citing This Article：

MA Weir Ling, ZHANG Weir Bin, ZHANG YuFei. Calbindin D28K containing neurons receivirng visceral and somatic nociceptive information in interstitial nucleus of the spinal trigeminal tract project to the parabrachial nuclei in the ratt. Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).