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Role of unsaturated fatty acids in the enhancemernt of muscarinic currernt by hypos motic membrane stretch i n guinea pig smooth muscle cells

CUI Yi- Feng, LI Lin, YU Yong Chun, JIN Zheng Yuan, LI Zai- Liu, XU Wen Yie

Department of Physiology, Yanbian University College of Medicine, and Center of

Abstract

To investigate the function of exogenous unsaturated fatty acids in hypos motic me mbrane stretch enhance ment of muscarinic current(lCCe) in antral circular smooth muscle cells of guinea pig，we recorded the membrane current with the conventional whole cell patch-clamp technique .lCCe elicited by 50 umol/ L carbachol(CCh) at the holding potential of一20 m V under isos motic
condition was taken as control .Hypos motic membrane stretch increased Iccb to 226. 0士21.0%.When the cells were pretreated with 5 umol/ L arachidonic acid(AA)，linoleic acid(LA) or oleic acid(OA)，lCCe was inhibited to 3. 8士0. 6%，35.2士0. 8%and 66. 6士0. 6%respectively .Hypos motic membrane stretch increased Iccb to 106.0士2. 5%，173. 2士6. 8%and 222.1士11 .0% of the control respectively .Five umol/ L AA inhibited hypos motic membrane stretch- enhanced Iccb by 51.2士3. 8%，while the control Iccb under isosmotic condition was inhibited by 96. 2士1 .6%.The results suggest that unsaturated fatty acids inhibited Iccb and the inhibitory effect is more significant when the unsaturation degree is increased .However，the unsaturated fatty acids are
not involved in the increase of Iccb induced by hypos motic membrane stretch.

Key words: unsaturated fatty acids;muscarinic current;hypos motic stretch;gastric antral myocytes;guinea pig

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Citing This Article：

CUI Yi- Feng, LI Lin, YU Yong Chun, JIN Zheng Yuan, LI Zai- Liu, XU Wen Yie. Role of unsaturated fatty acids in the enhancemernt of muscarinic currernt by hypos motic membrane stretch i n guinea pig smooth muscle cells . Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).