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Effect of interleukirr 2 on the activity of Ca~(2十)ATPase and Na~(十)/K~(十)ATPase of sarcoplas mic reticulum and sarcolemma

CAO Chuir Mei, XIA Qiangl, FU Chen, JIANG Hui-Dig, YE Zhi Guo, SHEN Yue- Liang, CHEN Jun-Zhu

Department of Physiology，Department of Cardiology，Xijing Hospital，and 3 Department of Physics，The Fourth Military Medical University, Xian 710032-

Abstract

The purpose of the present study was to investigate whether interleukixr2(IL 2) changes the activity of sarcoplas mic reticulu m(S R) Ca~(2十)ATPase，sarcole m mal Cat十ATPase and Na~(十)/K~(十)ATPase by measuring the Pi liberated from ATP hydrolysis with colorimetrical methods .It was shown
that the activity of Ca~(十)AT Pase in SRfrom IL 2-perfused(10，40，200，800 U/ ml) rat heart increased dose- dependently .After incubation of the SR with ATP(0 .1一4 m mol/ L)，the activity of S R Ca~(十)ATPase increased dosedependently in the control group .In the SR from 200 U/ ml IL 2-perfused
hearts，the activity of Ca~(十)ATPase was much higher than that in the control group .On the other hand，incubation of the S R with Ca~(十)(1-40 p mol/ L) increased the activity of SR Ca~(十)ATPase in the control group .The activity of S R Ca~(十)ATPase of IL 2-perfused hearts was inhibited as the function to Cat十.Pretreatment with specific x-opioid receptor antagonist norBNI(10 nmol/ L) for 5 min attenuated the effect of IL-2(200 U/ ml) on the activity of S R Cat十AT Pase.After pretreatment with pertussis toxin (PTX，5 mg/ L) or U73122(5 p mol/ L)，IL -2 failed to increase SR Cat十ATPase activity.The activity
of S R Cat十ATPase was not changed by incubation of SR isolated from normal hearts with IL -2 .Per fusion of rat heart with IL -2 did not affect the activity of sarcole m mal Ca~(2十)ATPase and Na~(十)/K十AT Pase.
It is concluded that perfusion of rat heart with IL -2 increases the activity of SR Cat十ATPase dose-dependently，which is mainly mediated by cardiac x opioid receptor pathway including a PTX sensitive Gi7protein and phospholipase C .IL -2 increases the activity of SR Ca~(十)ATPase as the function to ATP，but inhibits the activity of S R Cat十ATPase as the function to Ca~(2十).IL -2 has no effect on the activity of sarcolemmal Cat~(十)ATPase and Na~(十)/K十AT Pase.

Key words: Physiology;inte rleukirr 2;spectrophotometry;Heart;sarcoplas micreticulu m;Ca~(2+)AT Pase;opioid receptors

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Citing This Article：

CAO Chuir Mei, XIA Qiangl, FU Chen, JIANG Hui-Dig, YE Zhi Guo, SHEN Yue- Liang, CHEN Jun-Zhu. Effect of interleukirr 2 on the activity of Ca~(2十)ATPase and Na~(十)/K~(十)ATPase of sarcoplas mic reticulum and sarcolemma. Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).