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EFFECTS OF NA AND 5一HT ON THE SPONTANEOUS AND EVOKED ACTIVITIES OF PURKINJE CELLS IN CEREBELLAR SLICE

HU WEI-HONG, WANG Jian-Jun, ZHANG MIN-YUE, YU QI-XIANG

Division of Physiology, Department of Biological Science and Technology, Nanjing University, Nan.jing 210093)

Abstract

Effects of NA and 5-HT perfusion on the spontaneous activity and evoked responses of the Purkinje cells (PCs) to stimulation of white matter were studied in the rat cerebellar slices. The results were as follows:(1)Perfusion with NA could elicit inhibitory，excitatory or biphasic responses of the spontaneously discharging PCs，with inhibitory response in dominance (79. 8%).5-HT perfusion could also exert either excitatory or inhibitory effect on PC spontaneous activity.(2)When NA and 5-HT were perfused successively，NA tended to inhibit while 5-HT tended to excite in 53. 8%of the tested cells.(3)Most of the complex spike (CS) and simple spike (SS) responses evoked by white matter stimulation could be potentiated by NA(68. 2%，57. 1%)or depressed by 5-HT(60. 0%，68. 2%).(4)On the same cell under successively perfusion with NA and 5-HT，the evoked CS and SS responses of most cells(60. Ooo，52. 9%)showed a tendency to be facilitated by NA and suppressed by 5-HT. These results suggest that aminergic afferent fibers，by releasing NA and 5-HT，may affect
PC's excitability and responsiveness to synaptic inputs from mossy and limbing fibers，thus playing an important role in the cerebellar function.

Key words: Cerebellar cortex;Purkinje cell;Noradrenaline;5-hydroxytrypamine;cerebellar aminergic afferent system;cerebellar slice

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Citing This Article：

HU WEI-HONG, WANG Jian-Jun, ZHANG MIN-YUE, YU QI-XIANG. EFFECTS OF NA AND 5一HT ON THE SPONTANEOUS AND EVOKED ACTIVITIES OF PURKINJE CELLS IN CEREBELLAR SLICE. Acta Physiol Sin 1996; 48 (6): (in Chinese with English abstract).