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ALTERATION OF CARDIAC #alpha#_(l)-ADRENERGIC RECEPTOR SUBTYPES IN CARDIAC INSUFFICIENT RAT

XU KAI-MING, ZHANG YOU-YI , QU PENG, ZHANG PING, Tian Bin, HAN QI-DE (CHIDE HAN)

(Institute of Vascular Medicine, The Third Hospital, Beijing Medical University, Beijing 100083)

Abstract

The cardiac insufficiency was produced by simultaneous constriction of abdominal aortae and renal artery in rat. Radioligand binding and reverse transcription- pol),merase chain reaction (RT-PCR) were used respectively to determine the alterations of cardiac a,-adrenergic receptor (a,-AR) subtypes at protein level and gene transcription level. The Scatchard analysis of 1zsI_BE 2254 saturation curves showed that in cardiac insufficient rat hearts the KD values (229士32 vs 195士15 pmol/L) and the Bm$X vat ues(102士12vs96士17 fmol/mg) were not significantly different from those of control hearts. In the insufficient rat hearts，the inhibition of a,一AR specific binding by
WB 4101 showed that the high affinity sites (a,A+a,) were increased from the 22士5%of the control to 51士7%(PLO. 01).The a,A-AR mRNA level was increased，
a,B-AR mRNA level decreased while a,D-AR unchanged. The results suggest that in cardiac insufficient rat hearts the subtype composition is altered as shown by increased a,A-AR，decreased a1B-AR and unchanged a,D-AR，although the total amount of a,-AR isunchanged.

Key words: cardiac insufficiency;Heart;#alpha#_(1)adrenergic receptor;receptor subtypes

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Citing This Article：

XU KAI-MING, ZHANG YOU-YI , QU PENG, ZHANG PING, Tian Bin, HAN QI-DE (CHIDE HAN). ALTERATION OF CARDIAC #alpha#_(l)-ADRENERGIC RECEPTOR SUBTYPES IN CARDIAC INSUFFICIENT RAT. Acta Physiol Sin 1996; 48 (5): (in Chinese with English abstract).