ISSN 0371-0874, CN 31-1352/Q

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EFFECT OF ANOXIC PRECONDITIONING ON PROTEIN KINASEC ACTIVITY IN NEONATAL RAT CARDIOMYOCYTES

LIU XIU HUA, PANG YONG ZHENG, TANG CHAO-SHU, SU JING-YI

(Beijing Institute of Cardiovascular Research, Beijing Medical University,1000083)

Abstract

In a model of anoxia /reoxygenation (A/R) of cultured rat cardiomyocytes,protection of cellular damage,activation of protein kinase C (PKC) and PKC-mediated protein phosphorylation by anoxic preconditioning (APC) can be demonstrated as shown by the increase of cell viability,attenuation of formation of lipid peroxides (MDA)and lowering of the leakage of lactate dehydrogenase (LDH) and protein from cells. The results also show that transient anoxia mimicked the outcomes of activation of PKC as
shown by increased incorporation of 31p,especially in the 66 kD and 31 kD protein fractions. Preincubation of cardiomyocytes with H, (an inhibitor of PKC)completely abolished these protective effects of transient anoxia. Protein phosphatase inhibitor OA mimicked the protective effects of A/R,while protein phosphatase activator BDM in duced a complete abolishment. In short,we conclude that the protective effect of APC
is medicated by activation of PKC.

Key words: hypoxic preconditioning;Cardiomyocyte;Protein kinase C;protein phosphorylation

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Citing This Article:

LIU XIU HUA, PANG YONG ZHENG, TANG CHAO-SHU, SU JING-YI. EFFECT OF ANOXIC PRECONDITIONING ON PROTEIN KINASEC ACTIVITY IN NEONATAL RAT CARDIOMYOCYTES . Acta Physiol Sin 1997; 49 (4): (in Chinese with English abstract).