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EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR ON ANOXIA/REOXYGENATION INJURY OF RAT NEONATAL CARDIOMYOCYTES

LIU XIu-HUA, PANG YONG-ZHENG, TANG CHAO-SHU, SU JING-Y1

Institute of Cardiovascular Resaarch, Beijing Medicad University,Beijing 100083；Laboratory of Badu Bxzechraologital Company, Betijing 100041)

Abstract

The effects of basic fibroblast growth factor (bFGF)on anoxia/ reoxygenation (A/R) injury and protein kinase C (PKC) activity were studied on a model of A/R injury of neonatal rat cardiomyocytes to investigate the possibility of its using as a substrate for pharmacological preconditioning. The data indicated that bFGF improved the viability of cardiomyocytes，lowered the deplection of ATP and leakage of intracellular lactate dehydrogenase (LDH) in a concentration-dependent manner. PKC inhibitor，Hcompletely abolished the protective effects. It was also found that bFGF directely activated PKC in cardiomyocytes in a time course similar to that in hypoxic preconditioning.
The data suggested that the protective effect of bFGF on cardiomyocyte A/R injury might be mediated by PKC.

Key words: Basic fibroblast growth factor;cardiomyocyte;preconditioning;protein kinase C

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Citing This Article：

LIU XIu-HUA, PANG YONG-ZHENG, TANG CHAO-SHU, SU JING-Y1. EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR ON ANOXIA/REOXYGENATION INJURY OF RAT NEONATAL CARDIOMYOCYTES . Acta Physiol Sin 1997; 49 (4): (in Chinese with English abstract).