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WHOLE-CELL RECORDINGS OF THE SUPRAOPTIC NUCLEUS NEURONS FROM RAT HYPOTHALAMIC SLICES IN VITRO

LIU QING-SONG, JIA YOU-SHENG, XIE ZUO-PING, Ju Gong

(Institute of Neuroscience, The Fourth Military Medical University, .Xi'an 710032)

Abstract

Fifty-two supraoptic nucleus neurons in rat slice preparations were studied using whole cell patch-clamp technique. The mean passive and active membrane properties were measured as follows:resting membrane potential，59士8 mV;input resistance，535士129 MQ;time constant，32士9 ms;amplitude of the action potentials，99士11mV;overshoot，37士13 mV(，，=39).Most of these neurons showed a prominent slow after- hyperpolarization potential or current in response to depolarizing pulse. In votageclamp condition，it was found that virtually all supraoptic neurons (n= 13) were invad ed by spontaneous synaptic inputs. Pharmacological experiments showed that the excitatory postsynaptic currents (EPSCs) were mediated by non-NMDA glutamate receptors，whereas inhibitory postsynaptic currents (IPSCs) by GABAA receptors.

Key words: supraoptic nucleus;Brain slice;whole cell patch-clamp;synaptic transmission

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Citing This Article：

LIU QING-SONG, JIA YOU-SHENG, XIE ZUO-PING, Ju Gong. WHOLE-CELL RECORDINGS OF THE SUPRAOPTIC NUCLEUS NEURONS FROM RAT HYPOTHALAMIC SLICES IN VITRO. Acta Physiol Sin 1997; 49 (4): (in Chinese with English abstract).