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STUDIES ON CHARACTERISTICS OF PROLIFERATION AND DIFFERENTIATION OF HEMATOPOIETIC STEM CELLS IN MICE

LIU CUN-REN , HE Fu-CHU, WU ZU-ZE

Institute of Developmental Bwk%，School of Life Sciences, ShandongBeijing Institute of Radiation Medicine, Beijing 100850)

Abstract

By using Y chromosome specific sex-determining region (Sry) as a new cytogenetis marker and PCR technique，the characteristics of proliferation and differentiation of hematopoietic stem cells in mice were studied. Bone marrow cells from male mice were injected into lethally-irradiated female mice，PCR results indicated that all of the CFUS were originated from donor. It was found that CFU-S was able to proliferate and differentiate into various hematopoietic cells in iwo during its transplantation. Whereas the
fibroblasts within donor CFU- S and the fibroblasts from bone marrow in recipient mouse reestablished by donor CFU一S were shown to be originated from the recipient.The above data demonstrated that CFC-S from bone marrow in mice possessed the characteristics of hematopoietic stem cells but was not prone to differentiate into fibroblasts.

Key words: hematouoietic stem cell;CFC-S;CFU-S;fibroblast; Sry;PCR

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Citing This Article：

LIU CUN-REN , HE Fu-CHU, WU ZU-ZE. STUDIES ON CHARACTERISTICS OF PROLIFERATION AND DIFFERENTIATION OF HEMATOPOIETIC STEM CELLS IN MICE. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).