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STUDIES ON THE ISOLATION，STRUCTURE IDENTIFICATION AND BIOLOGICAL FUNCTION OF TWO TUMOR CELL SUPPRESSORS OF HUMAN FETAL LIVER ORIGIN

ZHANG QING-LIN, WU ZU-ZE (WU CHUTSE), CAO JU-RONG, XUE HUI-HUA, PEI XUE-TAO, WANG LI-SHENG , XU LI, DU ZE-HAN, FENG RuI,

Beijing Institute of Radiation Medicine, Beijing 100850)

Abstract

Our previous work showed the existence of low molecular weight tumor suppres-
sors in human fetal tissues. In this paper，two tumor cell suppressors were isolated and purified from methanol extract of human fetal liver by C18 reversed-phase medium pressure chromatography，gel filtration on Sephadex LH-20，and high-performance liquid chromatography，directed by suppression of growth of HL-60 cells. The structures of the suppressors were identified to be 7-ketocholesterol and 7-(3-hydroxycholesterol. Under the condition of in vitro agar plate culture，7-ketocholesterol and 7-卜hydroxycholesterol showed preferentially inhibitory effects on the growth of both human and
murine leukemic cell lines including human HL-60 and murine S-180 cells，but less effective on the growth of normal human and murine bone marrow granulocyte
macrophage progenitors (CFU-GM)

Key words: human fetal liver;low molecular weight tumor suppressor;separation;structure identification;7-ketocholesterol;7#beta#-hydroxycholesterol

Received: 　　Accepted:

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Citing This Article：

ZHANG QING-LIN, WU ZU-ZE (WU CHUTSE), CAO JU-RONG, XUE HUI-HUA, PEI XUE-TAO, WANG LI-SHENG , XU LI, DU ZE-HAN, FENG RuI,. STUDIES ON THE ISOLATION，STRUCTURE IDENTIFICATION AND BIOLOGICAL FUNCTION OF TWO TUMOR CELL SUPPRESSORS OF HUMAN FETAL LIVER ORIGIN . Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).