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EFFECT OF INTRAHIPPOCAMPAL MICROINJECTION OF ENKEPHALIN ON CELLULAR IMMUNE FUNCTION AND BRAIN IL- I #alpha# GENE EXPERSSION IN RAT

WANG A JING, YANG YI Zheng, Wu YuanMING, XIE HONG, Hu MOU-XIAN, Gao Na, HONG J]A , SUN CAI-LONG

(Reseach Center of Exepremental Medicine Tongji Medical University Wuhan430030)

Abstract

The effect of intrahippocampal m.icroinjection of enkephalin on cellular immune function and hippocampal 1L- 1 u gene expression was studied in rats. The results showed that:(1)The proliferation activity of splenie lymphocytes stimulated by Con A was significantly increased with microinjection of Leu一enkephalin I p1(18 mmol/L) or Met-enkephalin I ucl(18 mmol/L)into bilateral hippocampus，but was inhibited by Lipopolysaccharide 1 41(50 ng/ul).The proliferative response of splenic lymphocytes to Leu-enkephalin still persisted after bilateral adrenalectomy. Low doses of Leu- or Met-enkephalin(10’。，10一‘工mol/L) were also capable of causing proliferative activi-
ty of the Con A- stimulated cultured splenic lymphocytes:、ritro.(2)Using RT-PCR technique，IL--1 a gene expression was detected in hippocampus 90 min after bilateral irrtrahippocampal injection of LPS. The LPS-induced IL- l u. gene expression in hippocarrr pus could no longer be detected 30 min after microinjection of Met一or Leu一enkephalin into hippocampus. The above results suggested that intrahippocampal enkephalin might play an important role in neur<〕一itnmunomodulation by enhancing the inhibition of 1L-lc. gene expression in hippocampal formation.

Key words: Enkephalin;Hippocampus;1L-l#alpha# gene expression;neuroimrnunomodulation

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Citing This Article：

WANG A JING, YANG YI Zheng, Wu YuanMING, XIE HONG, Hu MOU-XIAN, Gao Na, HONG J]A , SUN CAI-LONG. EFFECT OF INTRAHIPPOCAMPAL MICROINJECTION OF ENKEPHALIN ON CELLULAR IMMUNE FUNCTION AND BRAIN IL- I #alpha# GENE EXPERSSION IN RAT. Acta Physiol Sin 1996; 48 (4): (in Chinese with English abstract).