Issue Archive

ATP-ACTIVATED SINGLE ION CHANNEL AND ITS PROPERTIES IN CEREBRAL CORTICAL NEURONS OF NEONATAL RAT

REN JUN, CHEN ZHU-HUA , CHEN PEI-XI

(Department of Physiology, Sun Yat-Sen University of Medical Sciences, Csuangzhou 510089)

Abstract

In the present study，single channel currents were recorded from single neurons dissociated from the cerebral cortex of neonatal SD rat by the cell-attached and inside- out modes of patch clamp technique. ATP-activated channels with a conductance of 32 pS were often recorded and permeable to Na+，K+ and Cs+，but not to Cl-. Most of open time distribution histograms were fitted by two exponential components and few by one，whereas closed time distribution histograms were fitted by two exponential components. Both mean open time and open probability were independent of membrane potential，but the latter increased with increasing ATP concentration. The channel activity
was recorded only in the presence of pipette solution containing ATP，and its conductance and open probability were not affected by ACh N-receptor antagonist hexamethonium and mecamylamine. It is suggested that ATP-activated ion channels probably exist in the cerebral cortical neurons of neonatal rat.

Key words: :ATP;Ion channel;patch clamp;cerebral cortical neurons

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

REN JUN, CHEN ZHU-HUA , CHEN PEI-XI. ATP-ACTIVATED SINGLE ION CHANNEL AND ITS PROPERTIES IN CEREBRAL CORTICAL NEURONS OF NEONATAL RAT . Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).