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EFFECT OF THROMBIN ON CYTOSOLIC CALCIUM AND MEMBRANE POTENTIAL IN SINGLE HUMAN PLATELETS

PANG JIAN-XIN , SHAN CHUN-WEN

(Department可Pharnwcology, First Military Medical University, Qngzhou 510515)

Abstract

Fluo-3 and d;-BA-Cg (3)-loaded human platelets were immobilized on a fibrinogen-coated surface and the cytosolic free [Ca2+]、and the membrane potentials were measured in single thrombin-activated platelets by ACAS-570 (Adherent Cell Analysis and Sorting Cytometer ).Fluorescence of fluo-3 and d;-BA-C4 (3) was low in rest in-f7 mobilized platelets without obviously repetitive spiking. When the platelets were activated by thrombin，a rapid rise of cytosolic ECa2+I; and membrane potential was observed.Subsequently，oscillations in〔Ca2+]，to an extent of about 500 fluorescent units were
observable，whereas the membrane potential remained at the peak level，i. e，the change of[CaZ+」，in no way of accord with that membrane potential. The above results indicate that the thrombin induced membrane depolarization is not due to influx of Ca2+.

Key words: hrombin;single platelet;cytosolic free-「CaZ+;membrane potential

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Citing This Article：

PANG JIAN-XIN , SHAN CHUN-WEN. EFFECT OF THROMBIN ON CYTOSOLIC CALCIUM AND MEMBRANE POTENTIAL IN SINGLE HUMAN PLATELETS. Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).