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IMPAIRMENT IN THE PHOSPHORYLATION OF Ca~(2+)一TRANSPORT ATPase OF RAT LIVER ENDOPLASMIC RETICULUM DURING SEPSIS

GAO AI-GUO, DONG LIN-WANG, WANG XUE-QING, LIU NAI-KUI , TANG CHAD-SHU, LIU MAW-SHUNG

Laboratory of Shock Research, Institute of Cardiovascular Research Beijing Medical University, Beijing 100083(Department of Physiology and Pharmacology, St. Louis University School of Medicine))

Abstract

The phosphorylation of Cat+-transport ATPase of rat liver endoplasmic reticulum (ER)during early and late septic shock induced by cecum ligation and puncture (CLP) was investigated by determining incorporation of [y-"P] ATP into Cat+-ATP phosphoprotein intermediate. Hepatic endoplasmic reticulum was isolated by differential centrifugation with sucrose density gradient. The Cal+-ATPase phosphoprotein intermediate was identified by SDS-PAGE. The results showed that the phosphorylation of Cat+-ATPase(115 kD) was decreased respectively by 15-23%(P<0. 05) and 17一27%(P<0. 05) at 9 h (early sepsis) and 18 h (late sepsis)，following the CLP in the rough，intermediate and smooth ER preparations. Kinetic analysis using rough ERshowed that the Vm$X for Ca~(2+) and for ATP for the phosphorylation of Cal+一ATPase were decreased dramatically during early and late sepsis，but without changes in the Km values. These results demonstrate that the phosphorylation of the phosphoprotein inter-
mediate of Ca~(2+)ATPase in rat liver was impaired during different phases of sepsis.

Key words: shock;septic;endoplasmic reticulum;phosphorylation;dephosphorylatipn

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Citing This Article：

GAO AI-GUO, DONG LIN-WANG, WANG XUE-QING, LIU NAI-KUI , TANG CHAD-SHU, LIU MAW-SHUNG. IMPAIRMENT IN THE PHOSPHORYLATION OF Ca~(2+)一TRANSPORT ATPase OF RAT LIVER ENDOPLASMIC RETICULUM DURING SEPSIS. Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).