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EFFECTS OF SEROTONIN ON THE PROLIFERATION OF PULMONARY ARTERIAL SMOOTH MUSCLE CELL UNDER HYPOXIA

Liu Jian, WANG PEI-YONG, LUO DE-CHENG, YU ZHONG-HE, SUN BIN-YONG

(Department of Pathophymology, Third Military Medical College, Chongqang 630038

Abstract

The effects of hypoxia on the bovine pulmonary arterial smooth muscle cell (PASM) proliferation arid serotonin transporter gene expression were investigated using 3H-Thymidine incorporation，immunocytochemical staining and mRNA dot blot hybridyzation，the results were as follows，PASM 3H-Thymidine incorporation increased in response to anoxia for' 24 h (P<0. 05).The anoxia-induced increased in DNA synthesis of PASM was significantly stimulated by 5-HT(100，10 and 1 pmol/L) (P<0. 001)，but 5一HT did not increase 3H-TdR incorporation under normoxic condition.Immunocytochemical reaction of serotonin in PASM under normoxic condition was markedly stronger than that of anoxic PASM (P<0. 001).Dot blot analysis showed hypoxia increased serotonin transporter mRNA expression of PASM (P<0. 01).These results indicate that stimulation of PASM proliferation with enhanced serotonin trans porter gene expression and high consumption of 5-HT in hypoxia or/and anoxia may
take part in the development of hypoxic pulmonany hypertention.

Key words: Hypoxia;;;;

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Citing This Article：

Liu Jian, WANG PEI-YONG, LUO DE-CHENG, YU ZHONG-HE, SUN BIN-YONG. EFFECTS OF SEROTONIN ON THE PROLIFERATION OF PULMONARY ARTERIAL SMOOTH MUSCLE CELL UNDER HYPOXIA. Acta Physiol Sin 1997; 49 (3): (in Chinese with English abstract).