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Effect of chronic electrical stimulation of phrenic nerve at different frequencies on mRNA and protein expressionof skeletal DHPR_(#alpha#1) and RyRs in the diaphragm muscle of rabbits

Zhang Suiyang, Liu Gang, Wang Donglin, Guo Xianjian, Qian Guisheng

Institute of Respiratory Medicine of the New Bridge Hospital,the Third Military Medical College.Chongqing 400037

Abstract

The results showed that mRNA and protein expressions of DHPR_(#alpha#1) and RyR_(1) in 10 and 20 Hz groups were more significantly lower than those in the control group (P<0.01), but mRNA and protein expressions of DHPR_(#alpha#1) and RyR_(1) were significantly higher in 50 and 100 Hz groups than those in the control group (P<0.01); a lower level of mRNA expression of RyR_(2) was found in 10 and 20 Hz groups. It is suggested that the calcium release unit and the signal transduction mode between DHPR and RyRs were altered from conformational changes of linked proteins to Ca~(2+)-induced Ca~(2+) release (CICR) in the diaphragmatic muscle of rabbits after chronic low-frequency electrical stimulation of phrenic nerve for 5 weeks.

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Citing This Article：

Zhang Suiyang, Liu Gang, Wang Donglin, Guo Xianjian, Qian Guisheng. Effect of chronic electrical stimulation of phrenic nerve at different frequencies on mRNA and protein expressionof skeletal DHPR_(#alpha#1) and RyRs in the diaphragm muscle of rabbits. Acta Physiol Sin 2004; 56 (2): (in Chinese with English abstract).