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Effect of urotensin II on the nitric oxide production in neonatal rat cardiomyocytes

Li Ling, Yuan Wenjun, Pan Xiujie, Wang Weizhong, Qiu Jingwei, Tang Chaoshu

Department of Physiology, The Second Military Medical University.Shanghai(200433)；Institute of Cardiovascular Research, The First Hospital, Peking University

Abstract

The aim of this study was to investigate the effect of urotensin II (U II) on the nitric oxide (NO) production in cultured neonatal rat cardiomyocytes. The endothelial nitric oxide synthase (eNOS) mRNA expression was assessed by semi-quantitative reverse transcription-polymerase chain reaction. The activity of nitric oxide synthase (NOS) and NO content in cardiomyocytes were measured. The current results showed that U II inhibited eNOS mRNAj expression, the NOS activity and the No production of cardiomyocytes. U II (0.1μmol/L) inhibited the NOS activity and the NO production in cardiomyocytes in a time-dependent manner. These results suggest that the cardiovascular effect of U II might be partially associated with NO production in cultured neonatal rat cardiomyocytes.

Key words: Urotensins;Nitric oxide;Endothelial nitric oxide synthase;Cardiomyocytes;Reverse transcription-polymerase chain reaction

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Citing This Article：

Li Ling, Yuan Wenjun, Pan Xiujie, Wang Weizhong, Qiu Jingwei, Tang Chaoshu. Effect of urotensin II on the nitric oxide production in neonatal rat cardiomyocytes. Acta Physiol Sin 2002; 54 (4): (in Chinese with English abstract).