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The expression of nestin in ischemia-injured brain of adult rat

Liu Pengchong, Lu Shiduo, Huang Yalin, Sun Fengyan

National key laboratory of medical neurobiology, Shanghai Medical College, Fudan University.Shanghai(200032)

Abstract

Immunohistochemistry and double immunofluorescent labeling techniques combined with confocal laser scanning microscope analysis were used to investigate the characteristic spatial induction profile of nestin following a transient middle cerebral artery occlusion in adult rat brain. The results showed that nestin was induced in ischemic core at 1 day after reperfusion. In addition to ischemic core, the expression of nestin increased in peri-ischemic I, II and III regions at 3 days and 1 week, then it decreased and narrowed along the rim of ischemic core 2 weeks after reperfusion. Double immunofluorescent labeling showed that nestin positive cells were mostly co-stained with GFAP, a astrocyte marker, in peri-ischemic I region 3 days after reperfusion. At 2 weeks, however, nestin cells showed a long process and the cells double stained with nestin and NSE, a neuronal specific marker, increased in the ischemic brain. The results suggest that cerebral ischemic induces nestin expression in damage neurons, which might favor the neuroprotection against ischemic damage.

Key words: Cerebral ischemia;Double immunofluorescent labeling;Confocal laser scanning microscope;Nestin

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Citing This Article：

Liu Pengchong, Lu Shiduo, Huang Yalin, Sun Fengyan. The expression of nestin in ischemia-injured brain of adult rat. Acta Physiol Sin 2002; 54 (4): (in Chinese with English abstract).