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CoCl_(2)-induced enhancement of glucose transport activity in mediating hypoxic tolerance in cultured hippocampal neurons

Yu Shun, Fan Ming, Zhao Tong, Ding Aishi, Wang Fuzhuang

Beijing Institute of Geriatrics,Xuanwu Hospital of Capital University of Medical Sciences.Beijing 100053；China

Abstract

The results showed taht the 2-deoxy-D-[1-~(3)H] glucose uptake rate and the mRNA expressions of glucose transporters (GLUT1 and GLUT3) in the hippocampal neurons were significantly increased after a 24-hour pretreatment with CoCl_(2). The cell injury induced by 6-hour or 8-hour hypoxic exposure was also greatly reduced by CoCl_(2) pretreatment. The protective effect of CoCl_(2) on the neurons was largely abolished by cytochalasin B, a specific inhibitor of glucose transporters. The results suggest that CoCl_(2) can increase mRNA expressions of GLUT1 and GLUT3 and glucose transporter activity of the neurons, which may be an important mechanism for the increased tolerance of the neurons to hypoxia.

Key words: Hypoxia;CoCl_(2);Glucose transporter;Neuron

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Citing This Article：

Yu Shun, Fan Ming, Zhao Tong, Ding Aishi, Wang Fuzhuang. CoCl_(2)-induced enhancement of glucose transport activity in mediating hypoxic tolerance in cultured hippocampal neurons. Acta Physiol Sin 2002; 54 (6): (in Chinese with English abstract).