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Difference in action sites between mecamylamine and hexamethonium on nicotinic receptors of sympathetic neurons

Liu Wei, Zheng Jianquan, Liu Zhenwei, Li Lijun, Wan Qin, Liu Chuangui

88 Hospital of Jinan Military Region.Taian 271000,Shandong；China

Abstract

The IC_(50) of MEC and HEX for antagonizing the effect of 0.08 mmol/L nicotine was 0.0012 and 0.0095 mmol/L, respectively. Both MEC and HEX accelerated the desensitizatiopn of nicotinic receptors. Furthermore, by comparing their effects at holding potentials -30, -70 and -110 mV, it was indicated that their suppressing effect on the nicotine-induced currents was voltage-dependent. However, different from that of HEX, the inhibitory effect of MEC increased with administrating the mixture of MEC and nicotine at intervals of 3 min, indicating a use-dependent effect of MEC. It is concluded that the action site of MEC on nicotinic receptors of sympathetic neurons is different from that of HEX.

Key words: Nicotinic receptors;Patch clamp technique;Sympathetic neurons;Allosteric modulation;Antagonist

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Citing This Article：

Liu Wei, Zheng Jianquan, Liu Zhenwei, Li Lijun, Wan Qin, Liu Chuangui. Difference in action sites between mecamylamine and hexamethonium on nicotinic receptors of sympathetic neurons. Acta Physiol Sin 2002; 54 (6): (in Chinese with English abstract).