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Neomycin inhibits nicotinic receptor-mediated response in pheochromocytoma (PC12) cells

Shi Lijun, Wang Chunan

Department of Physiology,Beijing Medical College of PLA.Beijing 100071

Abstract

Neomycin is one of the aminoglycoside antibiotics, and on the cellular level it inhibits phospholipase C. The effects of neomycin on the acetylcholine (ACh)-induced current (I_(ACh)) were studied in pheochromocytoma (PC12) cells by using the whole-cell clamp technique. The I_(ACh) on PC12 cells proved to be generated through activation of the neuronal nicotinic receptor. ACh (30 #mu#mol/L) induced an inward current at a holding potential of -80mV. When the cells were applied with neomycin on the peak of I_(ACh) was observed. This effect was fast, reversible and concentration-dependent. Pretreatment with neomycin for 3-8 min had no influence on its inhibitory effect. Activation of protein kinase C by using an exogenous activator exerted an inhibitory action on I_(ACh). However, intracellular dialysis with a PKC inhibitor (PKCI 19-31, 0.1-5#mu#mol/L) did not affect the inhibitory effect of neomycin. The results obtained suggest that neomycin exerts an inhibitory effect on I_(ACh) without involvement of the blockage of phospholipase C.

Key words: Aminoglycosides;Nicotinic acetylcholine receptor;Pheochromocytoma cells;Patch clamp techniques

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Citing This Article：

Shi Lijun, Wang Chunan. Neomycin inhibits nicotinic receptor-mediated response in pheochromocytoma (PC12) cells. Acta Physiol Sin 2002; 54 (5): (in Chinese with English abstract).