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Changes in field excitatory postsynaptic potential and population spike in CA1 region of rat hippocampal slices following low-frequency stimulation

Liang Huawei, Shen Yueliang, Chen Zhixiong, Xia Qiang

Department of Physiology,Zhejiang University School of Medicine.Hangzhou 310031,Zhejiang

Abstract

The technique of extracellular recording was used and the changes in the slope of field excitatory postsynaptic potential (S-EPSP) and the amplitude of population spike (A-PS) were observed when homosynaptic long-term depression (LTD) was induced by low-frequency stimulation (LFS) in the CA1 region of rat hippocampal slices. After LFS of 900 pulses at 1 Hz was delivered, S-EPSP and A-PA were reduced by 35.4+-5.3% and 68.0+-7.2%, respectively. When LFS of 450 pulses at 1 Hz was delivered, S-EPSP and A-PS were reduced by 14.3+-2.3% and 36.8+-6.7%, respectively. In both groups, the change in A-PS was significantly greater than that in S-EPSP (P<0.01). The changes in both indexes in the group of 900 pulses were greater than those in the group of 450 pulses (P<0.05). High Mg~(2+) (4 mmol/L) could attenuate the synaptic transmission, but did not affect the induction of LTD. In the high Mg~(2+) medium, the change in A-PS induced by LFS was also markedly greater than that in S-EPSP (P<0.01). These results indicate that the level of homosynaptic LTD indued by LFS is dependent not only on the numbers of pulses of LFS delivered, but also on the selection of evaluating index.

Key words: Synaptic plasticity;Long-term depression;Hippocampus

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Citing This Article：

Liang Huawei, Shen Yueliang, Chen Zhixiong, Xia Qiang. Changes in field excitatory postsynaptic potential and population spike in CA1 region of rat hippocampal slices following low-frequency stimulation. Acta Physiol Sin 2002; 54 (5): (in Chinese with English abstract).