Issue Archive

Inhibitory effect of alveolar macrophages on the proliferation of pulmonary fibroblasts

Zhang Jiansong, Yu Fengjiang, Qu Shulin, Li Xiang

Department of Physiology,Hunan Medical School.Changsha 410006,Hunan

Abstract

The effect of alveolar macrophages (AM) harvested from Wistar rats by lung lavage on proliferation of human embryo pulmonary fibroblasts in culture was investigated. It was observed that supernatants of AM decreased the uptake of [~(3)H] TdR by teh pulmonary fibroblasts. The AM activated with opsonized zymosan (OPZ) showed a stronger inhibitory effect on fibroblast proliferation compared with inactivated AM. Following pretreatment with indomethacin, the inhibitory effect of AM was abolished and reversed to stimulatory effect on pulmonary fibroblast proliferation. The PGE content in AM supernatant was measured with radioimmunoassay. It was observed that the inhibitory effect of AM was highly correlated to prostaglandin (PGE) content in the supernatant of AM.

Key words: Alveolar macrophage;Pulmonary fibrosis;Cell culture;Proliferation;Prostaglandins E

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Zhang Jiansong, Yu Fengjiang, Qu Shulin, Li Xiang. Inhibitory effect of alveolar macrophages on the proliferation of pulmonary fibroblasts. Acta Physiol Sin 2002; 54 (3): (in Chinese with English abstract).