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Relationship between proliferation of vascular smooth muscle cells and PDGF-AA and PDGFR-#alpha# expression in SHRs

Jin J un, Zhu Shanjun, Zhu Zhiming, Yang Yongjian, Ding Gang

Department of Cardiology,Xingqiao Hospital,Third Military Medical University.Chongqing 400037；China

Abstract

To explore the role of platelet derived growth factor-AA (PDGF-AA) and PDGFR-#alpha# expression in the proliferation and hypertrophy of vascular smooth muscle cells (VSMCs) in spontaneously hypertension rats (SHR), protein expression of PDGF-AA, PDGFR-#alpha# and PDGFR-#beta# in SHR/Wistar-Kyoto (WKY-VSMC was observed by Western blot. Proliferation and hypertrophy of SHR-VSMCs induced by PDGF-AA were observed by measurement of PCNA and [~(3)H] incorporation. PDGF-AA and PDGFR-#alpha# expression was markedly increased in SHR-VSMCs compared with that in WKY-VSMCs, but PDGFR-#beta# was not different in SHR and WKY-VSMCs. PDGF-AA induced PCNA expression and [~(3)H] incorporation was increased in a dose-dependent manner in SHR, but not in WKY.

Key words: Spontaneously hypertension;Platelet-derived growth factor;Platelet-derived growth factor-#alpha# receptor

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Citing This Article：

Jin J un, Zhu Shanjun, Zhu Zhiming, Yang Yongjian, Ding Gang. Relationship between proliferation of vascular smooth muscle cells and PDGF-AA and PDGFR-#alpha# expression in SHRs. Acta Physiol Sin 2002; 54 (2): (in Chinese with English abstract).