Issue Archive

Effects of insulin on the distribution of actins in vascular smooth muscle cells in the process of proliferation via mitogen-activated protein kinase in vitro

Wang Xukai, Wang Yan, He Zuoyun, Liu Guangyao, Yang Chengming

Department of Cardiology,Daping Hospital,The 3rd Military Medical University.Chongqing 400042；China；Department of Cardiology,Xinqiao Hospital,The 3rd Military Medical University.Chongqing 400037

Abstract

We found that insulin treatment resulted in proliferation of the VSMCs and an increase in [~(3)H] TdR incorporation. Meanwhile, the activities and expression of MAPK increased significantly compared to the control group. These effects of insulin were blocked by PKC inhibitor. In addition, insulin caused a redistribution of the intracellular actins in VSMCs, which was also inhibited by PKC inhibitor. It is, therefore, suggested that these effects of insulin on VSMCs proliferation and distrubution of the intracellular actins may be mediated by the MAPK signal transduction pathway.

Key words: Protein kinase C;Vascular smooth muscle cells;Cytoskeleton;Actins

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Wang Xukai, Wang Yan, He Zuoyun, Liu Guangyao, Yang Chengming. Effects of insulin on the distribution of actins in vascular smooth muscle cells in the process of proliferation via mitogen-activated protein kinase in vitro. Acta Physiol Sin 2002; 54 (2): (in Chinese with English abstract).