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The activation of vagus afferent in response to lipopolysaccharide: The role of interleukin-1

Lu Xiuying, Yang Guizhen, Sun Huichen

Norman Bethune National Peace Hospital.Shijiazhuang 050082,Hebei；China

Abstract

To study the possibility of activation of vagus afferent in response to lipopolysaccharide (LPS) through interleukin-1 (IL-1), Wistar rats were randomly divided into LPS group and saline (NS) group. The expression of c-Fos, CD14 and Mac-1 were detected by immunohistochemistry staining. IL-1 bioactivity was determined by L929 cell proliferation. The expression of IL-1R I mRNA was detected by in situ hybridization. Our results showed that there were some c-Fos protein expression positive neurons in the nodose ganglion in LPS group, but no c-Fos protein expression positive neurons in the nodose ganglion in control group. The number of macrophages (M#phi#) in the connective tissue surrounding the abdominal vagus increased significantly in response to LPS i.p. Forty-five minutes after the application of LPS, the IL-1 bioactivity in the supernatant of M#phi# was increased. Positive IL-1 R I mRNA neurons were also observed in the nodose ganglion in the LPS group.

Key words: Vagus nerve;c-Fos;Nodose ganglion;IL-l;IL-l R I

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Citing This Article：

Lu Xiuying, Yang Guizhen, Sun Huichen. The activation of vagus afferent in response to lipopolysaccharide: The role of interleukin-1. Acta Physiol Sin 2002; 54 (2): (in Chinese with English abstract).