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Recombinant human interleukin-10 inhibits vascular smooth muscle cell proliferation induced by TNF-#alpha#

Ouyang Ping, Peng Lisheng, Yang Hong, Wu Wenyan, Xu Anlong

College of Life Science,Zhongshan University.Guangzhou 510275,Guangdong

Abstract

Vessel injury provokes a release in proinflammatory cytokines that influence vascular smooth muscle cell (VSMC) proliferation. The purposes of this study was to determine the effects of recombinant human interleukin-10 (rhIL-10) on rat vascular smooth muscle cell proliferation and the activity of p44/p42 mitogen-activated protein kinase (MAPK) promoted by tumor necrosis factor-#alpha# (TNF-#alpha#). Rat aortic VSMCs were cultured and treated with rhIL-10 or TNF-#alpha# respectively, and then cotreated with rhIL-10 and TNF-#alpha#. The proliferation of VSMCs was quantified by colormetric assay. Cell cycle analysis was performed by flow cytometry.

Key words: Vascular smooth muscle;Interleukin-10;Mitogen-activated protein kinase;Cell proliferation

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Citing This Article：

Ouyang Ping, Peng Lisheng, Yang Hong, Wu Wenyan, Xu Anlong. Recombinant human interleukin-10 inhibits vascular smooth muscle cell proliferation induced by TNF-#alpha#. Acta Physiol Sin 2002; 54 (1): (in Chinese with English abstract).