Issue Archive

Exogenous rhCNTF inhibits myoblast differentiation of skeletal muscle of adult human in vitro

Chen Xiaoping, Liu Hong, Liu Shuhong, Wu Yan, Wu Haitao, Fan Ming

Institute of Basic Medical Sciences,Academy of Military Medical Sciences.Beijing 100850

Abstract

To determine the role of recombinant human ciliary neurotrophic factor (rhCNTF) in myogenesis, we observed the effects of rhCNTF (0-10ng/ml) on myoblast differentiation of adult human in vitro. The results showed that compared with the control group, the groups of 2.5-10 ng/ml rhCNTF treatment significantly inhibited myoblast differentiation (P<0.01), and the inhibition was dose-dependent and reversible. Western blots also indicated that compared with the control group, the expression of myogenin and p21, markers of myoblast differentiation phase, was significantly reduced (P<0.01), while Myf5 and desmin, markers of myoblast proliferative phase, significantly increased (P<0.01) in the groups of 2.5-10 ng/ml rhCNTF treatment. These findings demonstrate that exogenous rhCNTF can reversibly inhibit differentiation but permits proliferation of adult human myoblasts in vitro.

Key words: Recombinant human ciliary neurotrophic factor （rhCNTF）;Myoblast differentiation;Proliferation;

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Chen Xiaoping, Liu Hong, Liu Shuhong, Wu Yan, Wu Haitao, Fan Ming. Exogenous rhCNTF inhibits myoblast differentiation of skeletal muscle of adult human in vitro. Acta Physiol Sin 2003; 55 (4): (in Chinese with English abstract).