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Endogenous peroxynitrite mediates lipopolysaccharide-induced injury in culturd puomonary artery endothelial cells

Gu Chenyong, Ling Yiling, Xu Xiaohu, Zhu Tienian, Cong Bin

Shantou University Medical College.Shantou 515031,Guangdong；China

Abstract

LPS (1, 5 and 10 #mu#g/ml) caused a marked increase in fluorescent intensity of NT in a dose-dependent manner, which was significantly increased compared to the vehicle group (P<0.01). The number and percentage of NT positive cells were markedly increased (both P<0.05 vs vehicle group). Aminoguanidine (AG), a selective inhibitor of inducible nitric oxide synthase (iNOS), inhibited LPS-induced incrase in fluorescent intensity of NT in BPAECs. However, the number and percentage of NT positive cells had a tendency to reduce. LPS brought about an enhancement in MDA content and the activity of LDH in cultured supernatant. AG reversed the enhancement in MDA content induced by LPS (P<0.01). In contrast, AG had a marginal effect on the activity of LDH. LPS induced an increase in apoptotic rate in BPAECs in a dose-dependent manner. The number of apoptotic cells markedly increased as well. Some BPAECs stained with fluorescent probe ethidium bromide showed morphological features of apoptosis with chromatin condensation and nuclear fragmentation. AG reduced the apoptotic rate and the number of apoptotic cells, both of which were still higher than those of vehicle group (P<0.05).

Key words: Peroxynitrite lipopolysaccharide;Pulmonary;Artery;Endothelial cells

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Citing This Article：

Gu Chenyong, Ling Yiling, Xu Xiaohu, Zhu Tienian, Cong Bin. Endogenous peroxynitrite mediates lipopolysaccharide-induced injury in culturd puomonary artery endothelial cells. Acta Physiol Sin 2003; 55 (4): (in Chinese with English abstract).