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Arginine vasopressin-induced nitric oxide content changes in cultured cardiac fibroblasts and its relation to nuclear factor-#kappa#B

Fan Yanhong, Zhao Lianyou, Zheng Qiangsun, Xue Yusheng, Yang Xuedong, Tian Jianwei, Xu Lin

Department of Cardiology,Xijing Hospital,Fourth Military Medical University.Xi^an 710033,Shaanxi；China

Abstract

Nitric acid reductase method, spectrophotometry, reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescence-interactive laser cytometer techniques and Western blotting were used respectively to detect NO contents, NOS activity, iNOS mRNA expression and the activation of NF-#kappa#B in CFs. AVP increased NO contents, NOS activity and iNOS mRNA expressions in a concentration-dependent manner; NF-#kappa#B was activated and mobilized from cytoplasm to nucleus in AVP-induced CFs; PDTC, one of the inhibitors of NF-#kappa#B, could inhibit aforementioned increments. It is suggested that the increases in NO contents, elevation of NOS activity and increment of iNOS mRNA expression may be mediated through NF-#kappa#B activation pathway in cultured CFs induced by AVP, and that NF-#kappa#B is involved in the occurrence and development of myocardial fibrosis.

Key words: Arginine vasopressin;Nitric oxide;Cardiac fibroblasts;NF-κB

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Citing This Article：

Fan Yanhong, Zhao Lianyou, Zheng Qiangsun, Xue Yusheng, Yang Xuedong, Tian Jianwei, Xu Lin. Arginine vasopressin-induced nitric oxide content changes in cultured cardiac fibroblasts and its relation to nuclear factor-#kappa#B. Acta Physiol Sin 2003; 55 (4): (in Chinese with English abstract).