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Inhibition of moderate hypoxia-induced protein synthesis by vasonatrin peptide in cultured neonatal rat cardiomyocytes

Lu Shunyan, Zhu Miaozhang, Guo Haitao, Yu Jun, Wei Qiming

Department of Physiology,The Fourth Military Medical University.Xi^an 710032,Shaanxi

Abstract

The present work was to investigate the effects of vasonatrin peptide (VNP) on cardiomyocyte protein synthesis induced by moderate hypoxia. In cultured neonatal rat cardiomyocytes, MTT methods, total protein measurement and ~(3)H-leucine incorporation were used to calculate the cell number and measure the protein synthesis of cardiomyocytes. Furthermore, radioimmunoassay was undertaken to observe the effects of VNP on the intracellular levels of cAMP, cGMP and the concentration of endothelin (ET) in the culture medium.

Key words: Vasonatrin peptide(VNP);Hypoxia;Protein synthesis;Adenosine 3',5'-cyclic monophosphate (cAMP);Guanosine 3',5'-cyclic monophosphate (cGMP);Endothelin;Cardiomyocytes

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Citing This Article：

Lu Shunyan, Zhu Miaozhang, Guo Haitao, Yu Jun, Wei Qiming. Inhibition of moderate hypoxia-induced protein synthesis by vasonatrin peptide in cultured neonatal rat cardiomyocytes. Acta Physiol Sin 2002; 54 (1): (in Chinese with English abstract).