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Cholecystokinin octapeptide increases free intracellular calcium of guinea pig cardiomyocytes through activation of Ca~(2+) channel and tyrosine kinase

Zhao Xiaoyun, Ling Yiling, Shang Zhonglin, Li Qing, Yin Jingxiang, Tan Guojun

Hebei Provincial Geriatric Key Laboratory.Shijiazhuang 050051,Hebei；China；College of Biological Science,Hebei Normal University.Shijiazhuang 050017,Hebei

Abstract

The results obtained are as follows. (1) In the normal Tyrode^s solution containing 1.0 mmol/L Ca~(2+), CCK-8 (1--10~(4) pmol/L) eicited a rapid and marked increase in [Ca~(2+)]_(i)). (2) When cardiomyocytes were pretreated with the Ca~(2+) chelator EGTA (3 mmol/L) and Ca~(2+) channel antagonist nisoldipine (0.5 #mu#mol/L) for 5 min, CCK-8 (10~(2) pmol/L) caused a slow and small increase in [Ca~(2+)]_(i) (P<0.01). (3) Pretreatment with the nonselected CCK-receptor (CCK-R) antagonist proglumide (6 #mu#mol/L) or the tyrosine kinase inhibitor genistein (1 #mu#mol/L) for 5 min could inhibit the increase of [Ca~(2+)]_(i) induced by CCK-8 (10~(2) pmol/L) (P<0.01). The results suggest that CCK-8 increases the [Ca~(2+)]_(i) via activating the receptor-operated Ca~(2+) chanel and eliciting the influx of Ca~(2+) in isolated guinea pig cardiomyocytes, in which tyrosine kinase may be involved.

Key words: 八肽胆囊收缩素;心肌细胞;荧光强度;钙通道;共聚焦显微镜;受体

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Citing This Article：

Zhao Xiaoyun, Ling Yiling, Shang Zhonglin, Li Qing, Yin Jingxiang, Tan Guojun. Cholecystokinin octapeptide increases free intracellular calcium of guinea pig cardiomyocytes through activation of Ca~(2+) channel and tyrosine kinase. Acta Physiol Sin 2004; 56 (1): (in Chinese with English abstract).