Issue Archive

Effect of Chinese herb Tripterygium wilfordii Hook F monomer triptolide on apoptosis of PC12 cells induced by A#beta#1-42

Gu Ming, Zhou Huifang, Xue Bing, Niu Dongbin, He Qihua, Wang Xiaomin

Neuroscience Research Institute,Peking University.Beijing 100083

Abstract

It was found that 5 #mu#mol/L A#beta# decreased the cell viability to 66.3% and 50 #mu#mol/L A#beta# decreased it to 55.1%, significantly different from that of the control group. After treatment with 50 #mu#mol/L A#beta# for 48h, the apoptotic rate of PC12 cells increased obviously. The apoptotic rate was 5.37% in the control group, while after treatment with 0.5, 5 and 50 #mu#mol/L A#beta# for 48h, the apoptotic rate of PC12 cells went up to 10.19%, 8.02% and 16.63%, respectively. At the same time, the concentration of intracellular Ca~(2+) increased greatly after treatment with 50 #mu#mol/L A#beta# for 48h. At the concentration of 1×10~(-11) mol/L T_(10) remarkably inhibited the apoptosis induced by 50 #mu#mol/L A#beta#. In the naive group, the apoptotic rate was 4.83%. The apoptotic rate went up to 17.24% after treatment with 50 #mu#mol/L A#beta# for 48 h. After co-treatment with 1×10~(-11) mol/L T_(10) and 50 #mu#mol/L A#beta#, the apoptotic rate decreased to 8.91%, significantly different from that of the control group. At the same time, at the concentration of 1×10~(-11) mol/L T_(10) remarkably inhibited the increase of intracellular Ca~(2+) concentration induced by A#beta#. The results indicate that T_(10) has obvious protective effect on PC12 cells, which may be related to the inhibition of the cell apoptosis and increment of intracellular Ca~(2+) concentration induced by A#beta#.

Key words: Alzheimer's disease;P-amyloid;T_(10);neuroprotective effect

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Gu Ming, Zhou Huifang, Xue Bing, Niu Dongbin, He Qihua, Wang Xiaomin. Effect of Chinese herb Tripterygium wilfordii Hook F monomer triptolide on apoptosis of PC12 cells induced by A#beta#1-42. Acta Physiol Sin 2004; 56 (1): (in Chinese with English abstract).