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Binding between #alpha#_(1A)-adrenergic receptor and segment of bone morphogenetic protein-1 in human embryonic cell 293

Xu Qi, Zhang Tan, Han Qide, Zhang Youyi

Institute of Vascular Medicine,Peking University Third Hospital and Key Laboratory of Molecular Cardiology,Education Ministry.Beijing 100083

Abstract

In co-immunoprecipitation experiments, HEK293 cells expressing #alphla#_(1A)-AR or/and segment of BMP-1 were lysed and incubated with anti-FLAG M2 antibody, then the immunoprecipitation pellet was immunoblotted with either the HRP conjugated anti-FLAG antibody or the anti-HA antibody, which recognizes the HA-tagged segment of BMP-1. Segment of BMP-1 was present in the pellet immunoprecipitation of PDT-#alpha#_(1A) and PCP3HA co-transfected group. In conclusion, the results indicate that #alpha#_(1A)-AR and the segment of BMP-1 are present in the same complex in HEK293 cells.

Key words: Receptor adrenergic α1A;Bone morphogenetic proteins enzyme-linked;Immunosorbent assays

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Citing This Article：

Xu Qi, Zhang Tan, Han Qide, Zhang Youyi. Binding between #alpha#_(1A)-adrenergic receptor and segment of bone morphogenetic protein-1 in human embryonic cell 293. Acta Physiol Sin 2003; 55 (6): (in Chinese with English abstract).